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Chin. Opt. Lett.
 Home  List of Issues    Issue 11 , Vol. 16 , 2018    10.3788/COL201816.111801

Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging
Suhui Deng1;2, Yiping Xiao1, Jie Hu1, Jianfang Chen3, Yuhao Wang1, and Mingping Liu1
1 School of Information Engineering, [Nanchang University], Nanchang 330031 , China
2 Division of Physical Biology, [Shanghai Institute of Applied Physics, Chinese Academy of Sciences], Shanghai 201800, China
3 [Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences], Shanghai 201800, China

Chin. Opt. Lett., 2018, 16(11): pp.111801

Keywords(OCIS Code): 180.2520  170.6900  100.2980  110.0180  

The fluorescence from the out-of-focus region excited by the sidelobes of a Bessel beam is the major concern for light-sheet fluorescence microscopy (LSFM) with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of-focus blur, the improved image quality with the suppression of the Bessel beam sidelobes and enhanced sectioning ability with improved contrast are demonstrated.

Copyright: © 2003-2012 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Posted online:2018/10/31

Get Citation: Suhui Deng, Yiping Xiao, Jie Hu, Jianfang Chen, Yuhao Wang, and Mingping Liu, "Sidelobe suppression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging," Chin. Opt. Lett. 16(11), 111801(2018)

Note: This work was supported by the National Natural Science Foundation of China (Nos. 61665006, 61661028, 61565012, and 61378062) and the Natural Science Foundation of Jiangxi Province (Nos. 20161BAB212041, 20162BCB23012, and 20171ACB21018).


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