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Chin. Opt. Lett.
 Home  List of Issues    Issue 01 , Vol. 16 , 2018    10.3788/COL201816.013001

Single-turnover and multiple-turnover measurement of phytoplankton photosynthesis parameters using variable light pulse induced fluorescence
Chaoyi Shi1, Xianhe Gao1, Gaofang Yin2, Zhisong Qin2, Jingbo Duan2, Shuang Chen2, and Jun Lu1
1 Department of Electronic Information and Electrical Engineering, Hefei University, Hefei 230601 , China
2 State Environmental Protection Key Laboratory of Optical Monitoring Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 2 30031, China

Chin. Opt. Lett., 2018, 16(01): pp.013001

Keywords(OCIS Code): 300.6280  010.4450  120.0120  280.4788  

Using a measurement system based on fluorescence induced by variable pulse light, photosynthesis parameters of chlorella pyrenoidosa are obtained, employing single-turnover and multiple-turnover protocols under dark-adapted and light-adapted conditions. Under the light-adapted condition, σPSII′ is larger, and Fv′/Fm(ST)′ and Fv′/Fm(MT)′ are smaller than those of the dark-adapted condition, but the corresponding parameters possess good linear correlations. Fm(MT), Fm(MT)′, Fv/Fm(MT), and Fv′/Fm(MT)′, which are measured using the multiple-turnover protocol, are larger than those of the single-turnover protocol. The linear correlation coefficient between Fm(ST) and Fm(MT) is 0.984, and Fv/Fm(MT)=1.18Fv/Fm(ST). The linear correlation coefficient between Fm(ST)′ and Fm(MT)′ is 0.995, and Fv′/Fm(MT)′=1.36Fv′/Fm(ST)′.

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Posted online:2017/12/6

Get Citation: Chaoyi Shi, Xianhe Gao, Gaofang Yin, Zhisong Qin, Jingbo Duan, Shuang Chen, and Jun Lu, "Single-turnover and multiple-turnover measurement of phytoplankton photosynthesis parameters using variable light pulse induced fluorescence," Chin. Opt. Lett. 16(01), 013001(2018)

Note: This work was supported by the Natural Science Foundation of Anhui Province (No. 1708085QD87), the Open Fund of Qingdao National Laboratory for Marine Science and Technology (No. QNLM2016ORP0312), the Natural Science Foundation of Higher Education Institutions of Anhui Province (Nos. KJ2017A530 and KJ2016A594), the National Key Research and Development Plan (No. 2016YFC1400602), and the Natural Science Foundation of China (No. 31400317)


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