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Chin. Opt. Lett.
 Home  List of Issues    Issue 03 , Vol. 01 , 2003    Study on the violet LED-induced fluorescence spectra of thioredoxin reductase from human brain

Study on the violet LED-induced fluorescence spectra of thioredoxin reductase from human brain
Xiufeng Lan1, Tao Yang2, Shumei Gao1, Xiaosen Luo2, Zhonghua Shen1, Jian Lu1, Xiaowu Ni1, Lin Xu2
1 Department of Applied Physics, Nanjing University of Science & Technology, Nanjing 2100942 Department of Biochemistry, Nanjing Medical College, Second Military Medical University, Nanjing 210099

Chin. Opt. Lett., 2003, 01(03): pp.171-171-

Topic:Medical optics and biotechnology
Keywords(OCIS Code): 170.6280  300.6390  170.1530  

The technique of fluorescence spectroscopy is applied to study thioredoxin reductase (TrxR) in the cells of human brain. Experimental results show that, by the violet light emitting diode (LED, λmax=407 nm) light irradiation, TrxR is able to emit three striking spectral bands (528-582 nm; 588-660 nm; 683-700 nm). The fluorescence intensity is linear to the concentration of TrxR. The spectrum of denatured TrxR is rather different from that of organized TrxR, which reflects the structure change between denatured TrxR and organized TrxR. Furthermore, physical and biochemical mechanisms of fluorescence production for LED light-induced TrxR spectra and its characteristics are analyzed. This paper may be useful to better understand the structure of TrxR, and to provide new spectroscopic information to improve the resolution for this kind of biology structure.

Copyright: © 2003-2012 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Get Citation: Xiufeng Lan, Tao Yang, Shumei Gao, Xiaosen Luo, Zhonghua Shen, Jian Lu, Xiaowu Ni, Lin Xu, "Study on the violet LED-induced fluorescence spectra of thioredoxin reductase from human brain," Chin. Opt. Lett. 01(03), 171-171-(2003)

Note: X. Ni is the author to whom all correspondence should be addressed, his e-mail address is nxw@mail.njust.edu.cn.


1. J. R. Pedrajas, E. Kosmidou, A. Miranda-Vizuete, J.-A. Gustafsson, A. P.H. Wright, and G. Spyrou, J. Biol. Chem. 274, 6366 (1999).

2. G. Raddatz, V. I. Bunik, T. Scior, and H. Bisswanger, J. Mol. Model. 3, 359 (1997).

3. X. H. Qu, K. X. Huang, and H. B. Xu, Chemistry of Life (in Chinese) 19, 54 (1999).

4. L. Zhao and J. Gao, Progress in Physiological Sciences (in Chinese) 33, 74 (2002).

5. A.-K. Duhme, W. Meyer-Klaucke, D. J. White, et al., JBIC 4, 588 (1999).

6. N. S. Bhavesh, S. C. Panchal, and R. V. Hosur, Biochem. 40, 14727 (2001).

7. M. R. Thomas, D. Brown, S. Franzen, et al., Biochem. 40, 15407 (2001).

8. L. Brand and M. L. Johnson, Fluorescence Spectroscopy, Methods in Enzymology, Vol. 278 (Academic Press, London, 1997).

9. D. Millar, R. Hochstrasser, D. Chapman, and P. Youderian, Proc. SPIE 1640, 118 (1992).

10. T. Ha, A. Ting, J. Liang, et al., Proc. SPIE 3273, 179 (1998).

11. J. Chou, J. Y. Du, Y. Y. Feng, X. J. Yang, and T. H. Lu, Chin. J. Analytical Chemistry (in Chinese) 29, 219 (2001).

12. J. Fisher and J. R. P. Arnold, Instant Notes in Chemistry for Biologists (BIOS Scientific Publishers Limited, the United Kingdom, 1999) p. 217.

13. G. Z. Chen, X. Z. Huang, J. G. Xu, Z. X. Zheng, and Z. B. Wang, Fluorescence Analysis (in Chinese) (the second edition) (Science in China Press, Beijing, 1990) p. 25.

14. P. C. Tuner, A. G. McLennan, A. D. Bates, and M. R. H. White, Instant Notes in Molecular Biology (photoengraving) (BIOS Scientific Publishers Limited, the United Kingdom, 2001) p. 18.

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